Oligoclonal antibodies are a set of monoclonal antibodies optimized for detection of rat or mouse primary antibodies as secondary antibodies.
There are several reasons to choose oligoclonal secondary antibodies, including:
1. To reduce background caused by cross-reactions by polyclonal antibodies
There are several situations in which both rat and murine immunoglobulins are existed. For example, rat monoclonals are frequently used as primary antibodies for detection of proteins expressed by mice, where intrinsic mouse immunoglobulins may be around. Another example is double staining assays in which rat antibodies are often used in combination with murine antibodies. To avoid high background, the anti-rat or anti-mouse secondary antibodies must not cross-react with rat or murine primary antibodies. However most secondary antibodies on market are polyclonals and are difficult to avoid cross-reactivity without thorough pre-absorbance with mouse or rat immunoglobulins.
Oligoclonal antibodies do not react with immunoglobulins of their own species. Therefore, there is little or no background caused by cross-reactivity when using them as secondary antibodies.
2. To keep high sensitivities as polyclonal antibodies
Compared to monoclonal antibodies, polyclonal antibodies often have higher sensitivities as they can recognize several epitopes on an antigen whereas monoclonal antibodies can only recognize one. The oligoclonal antibodies we develop are composed by several monoclonal antibodies. Therefore, they can recognize several epitopes and have high sensitivity that is comparable to the polyclonal antibodies.
3. To reduce batch variations of polyclonal antibodies
Polyclonal antibodies are from immunized animals. As immune responses various from one animal to another, batch variation is often seen in polyclonal antibodies. Our oligoclonal antibodies are composed by monoclonal antibodies that are purified from cell culture. As long as we keep each monoclonal antibody concentration unchanged, the batch variation will be not a problem.
Oligoclonal vs Polyclonal Secondary Antibody - ELISA Result
Oligoclonal vs Polyclonal Secondary Antibody - Western Blot Result
Article reproduced from Absea Biotechnology