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Anti-SELPLG Antibody

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ABclonal
A1660
Details
Description: Rabbit polyclonal antibody to SELPLG.
Category: Primary Antibodies
Applications: WB, IHC, IF
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: Q14242
Gene ID: 6404
Immunogen: Recombinant protein of human SELPLG
Protein Length: 412
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: CLA; CD162; PSGL1; PSGL-1
Purification: Affinity purification
Background: This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200

Target (Information from UniProt)
Function: A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Tissue Specificity: Expressed on neutrophils, monocytes and most lymphocytes.
Post-Translational Modification: Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Cellular Location: Membrane.

Images
Western Blot - Anti-SELPLG Antibody from ABclonal (A1660) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using SELPLG antibody.
Immunofluorescence - Anti-SELPLG Antibody from ABclonal (A1660) - Antibody Supply Service
Immunofluorescence analysis of HeLa cell using SELPLG antibody.

Product Code - A1660
Size Price Quantity
50µl £148
100µl £243
200µl £372

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