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Anti-USP25 Antibody

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ABclonal
A1477
Details
Description: Mouse monoclonal antibody to USP25.
Category: Primary Antibodies
Applications: WB
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: Q9UHP3
Gene ID: 29761
Immunogen: A synthetic peptide of human USP25
Protein Length: 1055
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: USP25; USP21
Purification: Affinity purification
Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000

Target (Information from UniProt)
Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
Sequence Similarities: Belongs to the peptidase C19 family.
Post-Translational Modification: Acetylated.
Cellular Location: Cytoplasm.

Images
Western Blot - Anti-USP25 Antibody from ABclonal (A1477) - Antibody Supply Service
Western blot analysis of BxPC-3 cell lysate using USP25 antibody.

Product Code - A1477
Size Price Quantity
100µl £243
200µl £372

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