|Description:||Rabbit polyclonal antibody to FTO.|
|Immunogen:||Recombinant protein of human FTO|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Background:||The exact function of FTO is not known. Studies in mice suggest that it may be involved in nucleic acid demethylation, and that its mRNA level is regulated by feeding and fasting. Studies of type 2 diabetes indicate FTO is related to diabetes susceptibility. Mutation in this protein has been associated with growth retardation, developmental delay, coarse facies, and early death (1).|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:100|
Target (Information from UniProt)
|Function:||Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).|
|Tissue Specificity:||Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.|
|Involvement in Disease:||Growth retardation, developmental delay, and facial dysmorphism: A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years.
Obesity: A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
|Sequence Similarities:||Belongs to the fto family.|
|Cellular Location:||Nucleus. Nucleus speckle.|
Western blot analysis of extracts of 293T cell line, using FTO antibody.