|Description:||Rabbit polyclonal antibody to CD14.|
|Applications:||WB, IHC, IF|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100|
|Immunogen:||A synthetic peptide of human CD14|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).|
|Tissue Specificity:||Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.|
|Post-Translational Modification:||N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.|
|Cellular Location:||Cell membrane. Secreted. Membrane raft. Golgi apparatus.
Secreted forms may arise by cleavage of the GPI anchor.
|Synonyms:||CD 14 Antibody
CD14 antigen Antibody
CD14 molecule Antibody
Monocyte differentiation antigen CD14 Antibody
Monocyte differentiation antigen CD14 urinary form Antibody
Monocyte differentiation antigen CD14, membrane-bound form Antibody
Myeloid cell specific leucine rich glycoprotein Antibody
Myeloid cell-specific leucine-rich glycoprotein Antibody
Western blot analysis of extracts of SW480 cell lines, using CD14 antibody.