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Anti-VWF Antibody

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ABclonal
A1335
Details
Description: Rabbit polyclonal antibody to VWF.
Category: Primary Antibodies
Applications: WB, IHC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P04275
Gene ID: 7450
Immunogen: A synthetic peptide of human VWF
Protein Length: 2813
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: VWD; F8VWF
Purification: Affinity purification
Background: The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:100

Target (Information from UniProt)
Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Tissue Specificity: Plasma.
Involvement in Disease: von Willebrand disease 1: A common hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 1 is characterized by partial quantitative deficiency of circulating von Willebrand factor, that is otherwise structurally and functionally normal. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma.

von Willebrand disease 2: A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in altered platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet-dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma.

von Willebrand disease 3: A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses.
Post-Translational Modification: All cysteine residues are involved in intrachain or interchain disulfide bonds.
Cellular Location: Secreted. Secreted > Extracellular space > Extracellular matrix.

Localized to storage granules.

Images
Western Blot - Anti-VWF Antibody from ABclonal (A1335) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cell line, using VWF antibody.

Product Code - A1335
Size Price Quantity
50µl £148
100µl £243
200µl £372

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