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Anti-APOB Antibody

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ABclonal
A1329
Details
Description: Mouse monoclonal antibody to APOB.
Category: Primary Antibodies
Applications: WB, ICC, FC
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P04114
Gene ID: 338
Immunogen: Recombinant protein of human APOB
Protein Length: 4563
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: FLDB; LDLCQ4
Purification: Affinity purification
Background: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, ICC 1:500 - 1:1000, FC 1:50 - 1:200

Target (Information from UniProt)
Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in Disease: Hypobetalipoproteinemia, familial, 1: A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia.

Familial ligand-defective apolipoprotein B-100: Dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors.
Post-Translational Modification: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
Cellular Location: Cytoplasm. Secreted.

Images
Western Blot - Anti-APOB Antibody from ABclonal (A1329) - Antibody Supply Service
Western blot analysis of extracts of Recombinant protein, using APOB antibody.

Product Code - A1329
Size Price Quantity
50µl £199
100µl £307
200µl £452

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