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Anti-AGTR1 Antibody

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ABclonal
A1313
Details
Description: Rabbit polyclonal antibody to AGTR1.
Category: Primary Antibodies
Applications: WB, IHC, IF, FC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P30556
Gene ID: 185
Immunogen: A synthetic peptide of human AGTR1
Protein Length: 359
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1A; AGTR1B; AT2R1A; AT2R1B
Purification: Affinity purification
Background: Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLC? is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:100, IF 1:20 - 1:50, FC 1:20 - 1:50

Target (Information from UniProt)
Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue Specificity: Liver, lung, adrenal and adrenocortical adenomas.
Involvement in Disease: Renal tubular dysgenesis: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.
Post-Translational Modification: C-terminal Ser or Thr residues may be phosphorylated.
Cellular Location: Cell membrane.

Images
Western Blot - Anti-AGTR1 Antibody from ABclonal (A1313) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using AGTR1 antibody.

Product Code - A1313
Size Price Quantity
100µl £243
200µl £372

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