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Anti-PAI-1 Antibody

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ABclonal
A1297
Details
Description: Rabbit polyclonal antibody to PAI-1.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P05121
Gene ID: 5054
Immunogen: A synthetic peptide of human SERPINE1
Protein Length: 402
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: SERPINE1; PAI; PAI-1; PAI1; PLANH1; Plasminogen activator inhibitor1; Endothelial plasminogen activator inhibitor; Serpin E1
Purification: Affinity purification
Background: PAI-1 is a secreted protein that belongs to the serine proteinase inhibitor (serpin) superfamily. It inhibits urokinase and tissue plasminogen activators (uPA and tPA) and thus, reduces the conversion of inactive plasminogen to plasmin (1). PAI-1 regulates fibrinolysis and plays an important role in vessel patency and tissue remodeling. Secreted PAI-1 interacts with the extracellular matrix (ECM) component vitronectin, thereby modulating cell-ECM interactions (2,3). PAI-1 is expressed in a variety of tissues with higher expression in liver, vascular endothelial cells, platelets, macrophages, and adipose tissue (1). Increased levels of PAI-1 are associated with deep vein thrombosis (4). Defects in PAI-1 cause plasminogen activator inhibitor-1 deficiency (PAI-1D), which is characterized by increased bleeding after injury or surgery (5). Research studies have shown that high levels of PAI-1 are associated with obesity, aging, insulin resistance, and type 2 diabetes (6-8). PAI-1 is transcriptionally regulated by TGF-β and mediates TGF-β-induced inhibition of cell migration and invasion in cancer cells (9). Studies have shown PAI-1 to be also involved in fibrosis (10).
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000

Target (Information from UniProt)
Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Tissue Specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in Disease: Plasminogen activator inhibitor-1 deficiency: A hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Sequence Similarities: Belongs to the serpin family.
Post-Translational Modification: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.
Cellular Location: Secreted.

Images
Western Blot - Anti-PAI-1 Antibody from ABclonal (A1297) - Antibody Supply Service
Western blot analysis of extracts of various cells, using SERPINE1 antibody.

Product Code - A1297
Size Price Quantity
100µl £243
200µl £372

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