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Anti-RACK1 Antibody

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ABclonal
A1275
Overview
Name: Anti-RACK1 Antibody
Description: Rabbit polyclonal antibody to RACK1.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:100
Reactivity: Human
Immunogen: A synthetic peptide of human GNB2L1
Protein Length: 317
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.
Tissue Specificity: In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.
Sequence Similarities: Belongs to the WD repeat G protein beta family.
Post-Translational Modification: Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC.
Cellular Location: Cell membrane. Cytoplasm. Cytoplasm > Perinuclear region. Nucleus. Perikaryon. Cell projection > Dendrite. Cell projection > Phagocytic cup.

Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1 (PubMed:17956333). Also associated with the membrane in oncogene-transformed cells (PubMed:11884618). PKC activation induces translocation from the perinuclear region to the cell periphery (PubMed:11279199). In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei (By similarity). Localized to phagocytic cups following infection by Y.pestis (PubMed:21347310).
UniProt: P63244
Gene ID: 10399
Synonyms: Cell proliferation-inducing gene 21 protein Antibody
G protein beta 2 subunit Antibody
G protein subunit beta 2 Antibody
G protein subunit beta-2 Antibody
GBB2_HUMAN Antibody
GBLP_HUMAN Antibody
GNB2 Antibody
Gnb2-rs1 Antibody
Gnb2l1 Antibody
Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 Antibody
Guanine nucleotide binding protein beta 2 subunit Antibody
Guanine nucleotide binding protein beta polypeptide 2 like 1 Antibody
Guanine nucleotide binding protein beta subunit 2 like 1 Antibody
Guanine nucleotide binding protein beta subunit like protein 12.3 Antibody
Guanine nucleotide binding protein G I G S G T beta 2 subunit 2 Antibody
Guanine nucleotide binding protein G protein beta polypeptide 2 Antibody
Guanine nucleotide binding protein subunit beta 2 like 1 Antibody
Guanine nucleotide binding protein subunit beta like protein 12.3 Antibody
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 Antibody
Guanine nucleotide-binding protein subunit beta-2-like 1 Antibody
Guanine nucleotide-binding protein subunit beta-like protein 12.3 Antibody
H12.3 Antibody
HLC-7 Antibody
Human lung cancer oncogene 7 protein Antibody
lung cancer oncogene 7 Antibody
OTTHUMP00000174601 Antibody
OTTHUMP00000174602 Antibody
OTTHUMP00000223704 Antibody
OTTHUMP00000223870 Antibody
OTTHUMP00000223891 Antibody
OTTHUMP00000223893 Antibody
OTTHUMP00000223900 Antibody
OTTHUMP00000223902 Antibody
OTTHUMP00000223930 Antibody
OTTHUMP00000223931 Antibody
PIG21 Antibody
Proliferation inducing gene 21 Antibody
Protein homologous to chicken B complex protein guanine nucleotide binding Antibody
Receptor for activated C kinase Antibody
Receptor for activated C kinase 1 Antibody
Receptor of activated protein kinase C 1 Antibody
Signal transducing guanine nucleotide binding regulatory protein beta Antibody
Transducin beta chain 2 Antibody

Images
Western Blot - Anti-RACK1 Antibody from ABclonal (A1275) - Antibody Supply Service
Western blot analysis of extracts of HT-29 cells, using GNB2L1 antibody.

Product Code - A1275
Size Price Quantity
100µl £243
200µl £372

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