|Description:||Rabbit polyclonal antibody to SENP1.|
|Immunogen:||A synthetic peptide of human SENP1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||SENP1; SuPr-2; Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1|
|Background:||SUMO (small ubiquitin-related modifier), a member of the ubiquitin-like protein family, regulates diverse cellular functions of a variety of target proteins, including transcription, DNA repair, nucleocytoplasmic trafficking and chromosome segregation. SUMO precursor proteins undergo cleavage of the residues after the “GG” region by SUMO-specific proteases in maturation. This cleavage of the precursor is a prerequisite for subsequent sumoylation. The sentrin-specific (or SUMO-specific) protease (SENP) proteins belong to the peptidase C48 family and include SENP1-3 and SENP5-8. SENP1, SENP2 and SENP3 degrade UBL1 and SMT3H2 conjugates and subsequently release the monomers from sumoylated substrates. HIPK2 is a desumoylation target for SENP1 which shuttles between the cytoplasm and the nucleus. Mutation analyses reveal that SENP1 contains the nuclear export sequence (NES) within the extreme carboxyl-terminal region, and SENP1 is exported to the cytoplasm in a NES-dependent manner. SENP2 has been implicated as a downregulator of CTNNB1 levels and may therefore be a modulator of the Wnt pathway. SUMO protease SENP3 reverses the sumoylation of MEF2 to augment its transcriptional and myogenic activities. SENP5 localizes to the nucleolus and preferentially processes SUMO-3. It is thought to play a role in mitosis and/or cytokinesis. SENP6 localizes to the cytoplasm and releases SUMO-1. Expression of SENP6 is higher in reproductive organs, indicating that it may mediate processes related to reproduction. SENP8 is involved in the release of sentrins.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:1000 - 1:2000, IHC 1:50 - 1:200|
Target (Information from UniProt)
|Function:||Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity. Deconjugates SUMO2 from MTA1. Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed:25406032).|
|Tissue Specificity:||Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.|
|Sequence Similarities:||Belongs to the peptidase C48 family.|
|Cellular Location:||Nucleus. Cytoplasm.
Shuttles between cytoplasm and nucleus.
Western blot analysis of extracts of various cell lines, using SENP1 antibody.