|Description:||Rabbit polyclonal antibody to F11R.|
|Applications:||WB, IHC, IF|
|Immunogen:||Recombinant protein of human F11R|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||F11R; CD321; JAM; JAM-1; JAM-A; JAM1; JAMA; JCAM; KAT; PAM-1|
|Background:||Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200|
Target (Information from UniProt)
|Function:||Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).|
|Tissue Specificity:||Expressed in endothelium, epithelium and leukocytes (at protein level).|
|Sequence Similarities:||Belongs to the immunoglobulin superfamily.|
|Cellular Location:||Cell junction > Tight junction. Cell membrane.
Localized at tight junctions of both epithelial and endothelial cells.
Western blot analysis of extracts of various cells, using F11R antibody.
Immunofluorescence analysis of HeLa cell using F11R antibody. Blue: DAPI for nuclear staining.