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Anti-Lin28B Antibody

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ABclonal
A11479
Overview
Name: Anti-Lin28B Antibody
Description: Mouse monoclonal antibody to Lin28B.
Applications: WB, IHC, IF
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:100
Reactivity: Human
Immunogen: Recombinant protein of human LIN28B
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Tissue Specificity: Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463).
Sequence Similarities: Belongs to the lin-28 family.
Cellular Location: Nucleus. Nucleus > Nucleolus. Cytoplasm.

Predominantly nucleolar (PubMed:22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064).
UniProt: Q6ZN17
Gene ID: 389421
Synonyms: CSDD 2 Antibody
CSDD2 Antibody
FLJ16517 Antibody
Lin 28 homolog B (C. elegans) Antibody
Lin 28.2 Antibody
Lin-28B Antibody
LN28B_HUMAN Antibody
Protein lin-28 homolog B Antibody

Images
Western Blot - Anti-Lin28B Antibody from ABclonal (A11479) - Antibody Supply Service
Western blot analysis of extracts of Lin28B cells, using LIN28B antibody.

Product Code - A11479
Size Price Quantity
100µl £307
200µl £452

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