|Description:||Rabbit monoclonal antibody to MLKL.|
|Immunogen:||Recombinant protein of human MLKL|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Background:||This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200|
Target (Information from UniProt)
|Function:||Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.|
|Sequence Similarities:||Belongs to the protein kinase superfamily.|
|Post-Translational Modification:||Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.|
|Cellular Location:||Cytoplasm. Cell membrane.
Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Western blot analysis of extracts of various cells, using MLKL antibody.
Immunohistochemistry of paraffin-embedded human tonsil using MLKL antibody at dilution of 1:100 (40x lens).