|Description:||Mouse monoclonal antibody to XAF1.|
|Applications:||WB, IF, IP|
|Dilutions:||WB 1:500 - 1:2000, IF 1:50 - 1:100, IP 1:20 - 1:50|
|Immunogen:||Recombinant protein of human XAF1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.|
|Tissue Specificity:||Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).|
|Cellular Location:||Cytoplasm. Nucleus. Mitochondrion.
Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
|Synonyms:||BIRC 4 binding protein Antibody
BIRC4 binding protein Antibody
BIRC4-binding protein Antibody
HSXIAPAF 1 Antibody
XAF 1 Antibody
XIAP associated factor 1 Antibody
XIAP-associated factor 1 Antibody
Western blot analysis of extracts of 293T cells, using XAF1 antibody.