|Description:||Rabbit monoclonal antibody to YAP1.|
|Applications:||WB, IHC, IF, IP, FC|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100, IP 1:20 - 1:50, FC 1:20 - 1:50|
|Immunogen:||Recombinant protein of human YAP1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).|
|Tissue Specificity:||Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).|
|Involvement in Disease:||Coloboma, ocular, with or without hearing impairment, cleft lip/palate, and/or mental retardation: An autosomal dominant disease characterized by uveal colobomata, microphthalmia, cataract and cleft lip/palate. Considerable variability is observed among patients, uveal colobomata being the most constant feature. Some patients manifest mental retardation of varying degree and/or sensorineural, mid-frequency hearing loss.|
|Sequence Similarities:||Belongs to the YAP1 family.|
|Post-Translational Modification:||Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation (PubMed:18158288, PubMed:20048001). Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage (PubMed:18280240). Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation (PubMed:20048001). Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1 (PubMed:21364637).|
|Cellular Location:||Cytoplasm. Nucleus.
Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271).
|Synonyms:||65 kDa Yes associated protein Antibody
65 kDa Yes-associated protein Antibody
YAp 1 Antibody
YAP 65 Antibody
YAP1 (phospho S127) Antibody
YAP1 (phospho Y357) Antibody
yes -associated protein delta Antibody
Yes associated protein 1 Antibody
Yes associated protein 1 65kDa Antibody
Yes associated protein 2 Antibody
yes associated protein beta Antibody
Yorkie homolog Antibody
Western blot analysis of extracts of HepG2 cells, using YAP1 antibody.
Immunohistochemistry of paraffin-embedded human kidney using YAP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using YAP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using YAP1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Hela cell using YAP1 antibody.
Immunofluorescence analysis of HepG2 cell using YAP1 antibody.