|Description:||Rabbit monoclonal antibody to Alas1.|
|Immunogen:||Recombinant protein of human ALAS1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||ALAS; MIG4; ALAS3; ALASH; ALAS-H|
|Background:||This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, ICC 1:50 - 1:200, IF 1:50 - 1:100|
Target (Information from UniProt)
|Sequence Similarities:||Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.|
|Cellular Location:||Mitochondrion matrix.|
Western blot analysis of extracts of various cells, using ALAS1 antibody.
Immunofluorescence analysis of MCF-7 cell using ALAS1 antibody.
Immunofluorescence analysis of Hela cell using ALAS1 antibody.
Immunofluorescence analysis of HepG2 cell using ALAS1 antibody.