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Anti-AMPK alpha 1 Antibody

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Name: Anti-AMPK alpha 1 Antibody
Description: Mouse monoclonal antibody to AMPK alpha 1.
Applications: WB, IHC, IF
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:100
Reactivity: Human
Immunogen: Recombinant protein of human PRKAA1
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Sequence Similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Post-Translational Modification: Ubiquitinated.
Cellular Location: Cytoplasm. Nucleus.

In response to stress, recruited by p53/TP53 to specific promoters.
UniProt: Q13131
Gene ID: 5562
Synonyms: 5 AMP activated protein kinase alpha 1catalytic subunit Antibody
5 AMP activated protein kinase catalytic alpha 1 chain Antibody
5' AMP activated protein kinase catalytic subunit alpha 1 Antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 Antibody
AAPK1 Antibody
AAPK1_HUMAN Antibody
ACACA kinase Antibody
acetyl CoA carboxylase kinase Antibody
AI194361 Antibody
AI450832 Antibody
AL024255 Antibody
AMP -activate kinase alpha 1 subunit Antibody
AMP-activated protein kinase, catalytic, alpha -1 Antibody
AMPK Antibody
AMPK 1 Antibody
AMPK alpha 1 (phospho S487) Antibody
AMPK alpha 1 (phospho S496) Antibody
AMPK alpha 1 (phospho T172 + T183) Antibody
AMPK alpha 1 chain Antibody
AMPK subunit alpha-1 Antibody
AMPK1 Antibody
AMPKa1 Antibody
AMPKalpha1 Antibody
C130083N04Rik Antibody
cb116 Antibody
EC Antibody
HMG CoA reductase kinase Antibody
HMGCR kinase Antibody
hormone sensitive lipase kinase Antibody
Hydroxymethylglutaryl CoA reductase kinase Antibody
im:7154392 Antibody
kinase AMPK alpha1 Antibody
MGC33776 Antibody
MGC57364 Antibody
OTTHUMP00000161795 Antibody
OTTHUMP00000161796 Antibody
PRKAA 1 Antibody
PRKAA1 Antibody
Protein kinase AMP activated alpha 1 catalytic subunit Antibody
SNF1-like protein AMPK Antibody
SNF1A Antibody
Tau protein kinase PRKAA1 Antibody
wu:fa94c10 Antibody

Western Blot - Anti-AMPK alpha 1 Antibody from ABclonal (A11101) - Antibody Supply Service
Western blot analysis of extracts of HeLa cells, using PRKAA1 antibody.

Product Code - A11101
Size Price Quantity
100µl £307
200µl £452

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