|Description:||Rabbit monoclonal antibody to CD8.|
|Applications:||WB, IHC, IF|
|Immunogen:||Recombinant protein of human CD8A|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||CD8; MAL; p32; Leu2|
|Background:||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100|
Target (Information from UniProt)
|Function:||Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.|
|Involvement in Disease:||CD8 deficiency, familial: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.|
|Post-Translational Modification:||All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.|
|Cellular Location:||Cell membrane.|
Western blot analysis of extracts of various cells, using CD8A antibody.
Immunohistochemistry of paraffin-embedded human spleen using CD8A antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Hela cell using CD8A antibody.
Immunofluorescence analysis of CRC cell using CD8A antibody.