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Anti-Active Caspase-3 Antibody

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ABclonal
A11021
Overview
Name: Anti-Active Caspase-3 Antibody
Description: Rabbit monoclonal antibody to Active Caspase-3.
Applications: WB, IHC, IF
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100
Reactivity: Human
Immunogen: Recombinant protein of human CASP3
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence Similarities: Belongs to the peptidase C14A family.
Post-Translational Modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
Cellular Location: Cytoplasm.
UniProt: P42574
Gene ID: 836
Synonyms: Apopain Antibody
CASP 3 Antibody
CASP-3 Antibody
CASP3 Antibody
CASP3_HUMAN Antibody
Caspase 3 Antibody
Caspase-3 subunit p12 Antibody
CPP 32 Antibody
CPP-32 Antibody
CPP32B Antibody
Cysteine protease CPP32 Antibody
PARP cleavage protease Antibody
Protein Yama Antibody
SCA-1 Antibody
SCA1 Antibody
SREBP cleavage activity 1 Antibody
Yama Antibody

Images
Western Blot - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Western blot analysis of extracts of various cells, using CASP3 antibody.
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunofluorescence analysis of PC-3M cell using CASP3 antibody.
Immunofluorescence - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunofluorescence analysis of Hela cell using CASP3 antibody.

Product Code - A11021
Size Price Quantity
100µl £372
200µl £565

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