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Anti-Active Caspase-3 Antibody

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ABclonal
A11021
Details
Description: Rabbit monoclonal antibody to Active Caspase-3.
Category: Primary Antibodies
Applications: WB, IHC, IF
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P42574
Gene ID: 836
Immunogen: Recombinant protein of human CASP3
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: CPP32; SCA-1; CPP32B
Purification: Affinity purification
Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100

Target (Information from UniProt)
Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence Similarities: Belongs to the peptidase C14A family.
Post-Translational Modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
Cellular Location: Cytoplasm.

Images
Western Blot - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Western blot analysis of extracts of various cells, using CASP3 antibody.
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunofluorescence analysis of PC-3M cell using CASP3 antibody.
Immunofluorescence - Anti-Active Caspase-3 Antibody from ABclonal (A11021) - Antibody Supply Service
Immunofluorescence analysis of Hela cell using CASP3 antibody.

Product Code - A11021
Size Price Quantity
100µl £372
200µl £565

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