Anti-Active Caspase-3 AntibodyDatasheet
|Description:||Rabbit monoclonal antibody to Active Caspase-3.|
|Applications:||WB, IHC, IF|
|Immunogen:||Recombinant protein of human CASP3|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||CPP32; SCA-1; CPP32B|
|Background:||This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100|
Target (Information from UniProt)
|Function:||Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.|
|Tissue Specificity:||Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.|
|Sequence Similarities:||Belongs to the peptidase C14A family.|
|Post-Translational Modification:||Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.|
Western blot analysis of extracts of various cells, using CASP3 antibody.
Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of PC-3M cell using CASP3 antibody.
Immunofluorescence analysis of Hela cell using CASP3 antibody.