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Anti-Ki67 Antibody

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ABclonal
A11005
Overview
Name: Anti-Ki67 Antibody
Description: Rabbit monoclonal antibody to Ki67.
Applications: WB, IHC, IF, IP, FC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100, IP 1:20 - 1:50, FC 1:20 - 1:50
Reactivity: Human
Immunogen: Recombinant protein of human MKI67
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Post-Translational Modification: Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Cellular Location: Chromosome. Nucleus. Nucleus > Nucleolus.

Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Biotechnological Use: Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
UniProt: P46013
Gene ID: 4288
Synonyms: Antigen identified by monoclonal Antibody
Antigen KI-67 Antibody
Antigen KI67 Antibody
KI67_HUMAN Antibody
KIA Antibody
Marker of proliferation Ki-67 Antibody
MIB Antibody
MIB 1 Antibody
MKI67 Antibody
PPP1R105 Antibody
Proliferation marker protein Ki-67 Antibody
Proliferation related Ki 67 antigen Antibody
Protein phosphatase 1 regulatory subunit 105 Antibody
RP11-380J17.2 Antibody

Images
Western Blot - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Western blot analysis of extracts of HepG2 cells, using MKI67 antibody.
Immunohistochemistry - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded mouse spleen using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human tonsil using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human spleen using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded mouse testis using MKI67 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Anti-Ki67 Antibody from ABclonal (A11005) - Antibody Supply Service
Immunofluorescence analysis of A431 cell using MKI67 antibody.

Product Code - A11005
Size Price Quantity
100µl £372
200µl £565

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