|Description:||Rabbit monoclonal antibody to Ki67.|
|Applications:||WB, IHC, IF, IP, FC|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100, IP 1:20 - 1:50, FC 1:20 - 1:50|
|Immunogen:||Recombinant protein of human MKI67|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).|
|Post-Translational Modification:||Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.|
|Cellular Location:||Chromosome. Nucleus. Nucleus > Nucleolus.
Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
|Biotechnological Use:||Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).|
|Synonyms:||Antigen identified by monoclonal Antibody
Antigen KI-67 Antibody
Antigen KI67 Antibody
Marker of proliferation Ki-67 Antibody
MIB 1 Antibody
Proliferation marker protein Ki-67 Antibody
Proliferation related Ki 67 antigen Antibody
Protein phosphatase 1 regulatory subunit 105 Antibody
Western blot analysis of extracts of HepG2 cells, using MKI67 antibody.
Immunohistochemistry of paraffin-embedded mouse spleen using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using MKI67 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MKI67 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cell using MKI67 antibody.