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Anti-c-Kit Antibody

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ABclonal
A10933
Overview
Name: Anti-c-Kit Antibody
Description: Rabbit monoclonal antibody to c-Kit.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:200
Reactivity: Human
Immunogen: Recombinant protein of human KIT
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Tissue Specificity: Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
Involvement in Disease: Piebald trait: Autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes.

Gastrointestinal stromal tumor: Common mesenchymal neoplasms arising in the gastrointestinal tract, most often in the stomach. They are histologically, immunohistochemically, and genetically different from typical leiomyomas, leiomyosarcomas, and schwannomas. Most GISTs are composed of a fairly uniform population of spindle-shaped cells. Some tumors are dominated by epithelioid cells or contain a mixture of spindle and epithelioid morphologies. Primary GISTs in the gastrointestinal tract commonly metastasize in the omentum and mesenteries, often as multiple nodules. However, primary tumors may also occur outside of the gastrointestinal tract, in other intra-abdominal locations, especially in the omentum and mesentery.

Testicular germ cell tumor: A common malignancy in males representing 95% of all testicular neoplasms. TGCTs have various pathologic subtypes including: unclassified intratubular germ cell neoplasia, seminoma (including cases with syncytiotrophoblastic cells), spermatocytic seminoma, embryonal carcinoma, yolk sac tumor, choriocarcinoma, and teratoma.

Leukemia, acute myelogenous: A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes.
Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Post-Translational Modification: Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Cellular Location: Cell membrane.
UniProt: P10721
Gene ID: 3815
Synonyms: C Kit Antibody
c-Kit (phospho Y568 + Y570) Antibody
c-Kit (phospho Y703) Antibody
c-Kit (phospho Y721) Antibody
c-Kit (phospho Y730) Antibody
c-Kit (phospho Y823) Antibody
c-Kit (phospho Y936) Antibody
c-Kit Ligand Antibody
CD117 Antibody
Kit Antibody
Kit Ligand Antibody
KIT oncogene Antibody
KIT proto oncogene receptor tyrosine kinase Antibody
KIT_HUMAN Antibody
Mast cell growth factor receptor Antibody
Mast/stem cell growth factor receptor Kit Antibody
MGF Antibody
p145 c-kit Antibody
PBT Antibody
Piebald trait protein Antibody
Proto oncogene c Kit Antibody
Proto oncogene tyrosine protein kinase Kit Antibody
Proto-oncogene c-Kit Antibody
SCF Receptor Antibody
SCFR Antibody
soluble KIT variant 1 Antibody
Steel Factor Receptor Antibody
Stem cell factor receptor Antibody
tyrosine protein kinase Kit Antibody
Tyrosine-protein kinase Kit Antibody
v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog Antibody
v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein Antibody
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog Antibody

Images
Immunohistochemistry - Anti-c-Kit Antibody from ABclonal (A10933) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human spleen using KIT antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-c-Kit Antibody from ABclonal (A10933) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human mammary cancer using KIT antibody at dilution of 1:100 (40x lens).

Product Code - A10933
Size Price Quantity
100µl £372
200µl £565

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