|Description:||Rabbit monoclonal antibody to ICAM1.|
|Applications:||WB, IHC, IF|
|Immunogen:||Recombinant protein of human ICAM1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||BB2; CD54; P3.58|
|Background:||This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100|
Target (Information from UniProt)
|Function:||ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.|
|Sequence Similarities:||Belongs to the immunoglobulin superfamily. ICAM family.|
|Post-Translational Modification:||Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.|
Western blot analysis of extracts of various cells, using ICAM1 antibody.
Immunohistochemistry of paraffin-embedded human spleen using ICAM1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using ICAM1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ICAM1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using ICAM1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HUVEC cell using ICAM1 antibody.
Immunofluorescence analysis of A431 cell using ICAM1 antibody.