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Anti-Glutathione Peroxidase 1 Antibody

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ABclonal
A10874
Details
Description: Mouse monoclonal antibody to Glutathione Peroxidase 1.
Category: Primary Antibodies
Applications: WB, IHC, IF, FC
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P07203
Gene ID: 2876
Immunogen: Recombinant protein of human GPX1
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: GPXD; GSHPX1
Purification: Affinity purification
Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants and multiple pseudogenes of this gene have been identified.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:100, FC 1:20 - 1:50

Target (Information from UniProt)
Function: Protects the hemoglobin in erythrocytes from oxidative breakdown.
Sequence Similarities: Belongs to the glutathione peroxidase family.
Post-Translational Modification: During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
Cellular Location: Cytoplasm.

Images
Western Blot - Anti-Glutathione Peroxidase 1 Antibody from ABclonal (A10874) - Antibody Supply Service
Western blot analysis of extracts of HepG2 cells, using GPX1 antibody.

Product Code - A10874
Size Price Quantity
100µl £307
200µl £452

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