phone icon
+44 (0)1223 298875

Anti-CD3 epsilon Antibody

file icon Datasheet
ABclonal
A10786
Details
Description: Rabbit monoclonal antibody to CD3 epsilon.
Category: Primary Antibodies
Applications: WB, IHC, IP, FC
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P07766
Gene ID: 916
Immunogen: Recombinant protein of human CD3E
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: T3E; TCRE; IMD18
Purification: Affinity purification
Background: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IP 1:20 - 1:50, FC 1:20 - 1:50

Target (Information from UniProt)
Function: The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
Involvement in Disease: Immunodeficiency 18: An autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood of recurrent infections. The severity is variable, encompassing both a mild immunodeficiency and severe combined immunodeficiency (SCID), resulting in early death without bone marrow transplantation in some patients. Immunologic work-up of the IMD18 SCID patients shows a T cell-negative, B cell-positive, natural killer (NK) cell-positive phenotype, whereas T-cell development is not impaired in the mild form of IMD18.
Cellular Location: Cell membrane.

Images
Western Blot - Anti-CD3 epsilon Antibody from ABclonal (A10786) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cells, using CD3E antibody.
Immunohistochemistry - Anti-CD3 epsilon Antibody from ABclonal (A10786) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human spleen using CD3E antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-CD3 epsilon Antibody from ABclonal (A10786) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human tonsil using CD3E antibody at dilution of 1:100 (40x lens).

Product Code - A10786
Size Price Quantity
100µl £372
200µl £565

or
Request a quotation
Contact us directly to order
  • call icon +44 (0)1223 298875
  • +44 (0)1223 281447
  • email icon orders@ab-y-ss.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe