|Description:||Rabbit monoclonal antibody to Caveolin-2.|
|Applications:||WB, IHC, IF, IP|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100, IP 1:20 - 1:50|
|Immunogen:||Recombinant protein of human CAV2|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).|
|Tissue Specificity:||Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.|
|Sequence Similarities:||Belongs to the caveolin family.|
|Post-Translational Modification:||Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin (By similarity).|
|Cellular Location:||Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > Caveola.
Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
Caveolae protein 20 kD Antibody
Caveolin 2 Antibody
Caveolin 2 isoform a and b Antibody
Caveolin-2 (phospho Y19) Antibody
Caveolin-2 (phospho Y27) Antibody
Western blot analysis of extracts of HUVEC cells, using CAV2 antibody.
Immunohistochemistry of paraffin-embedded human cervix using CAV2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using CAV2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Hela cell using CAV2 antibody.