|Description:||Rabbit monoclonal antibody to ATM.|
|Applications:||WB, IHC, IF|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:200, IF 1:50 - 1:100|
|Immunogen:||Recombinant protein of human ATM|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.|
|Tissue Specificity:||Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.|
|Involvement in Disease:||Ataxia telangiectasia: A rare recessive disorder characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. Patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.|
|Sequence Similarities:||Belongs to the PI3/PI4-kinase family. ATM subfamily.|
|Post-Translational Modification:||Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.|
|Cellular Location:||Nucleus. Cytoplasmic vesicle.
Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
|Synonyms:||A-T mutated Antibody
A-T mutated homolog Antibody
AT mutated Antibody
Ataxia telangiectasia mutated Antibody
Ataxia telangiectasia mutated gene Antibody
Ataxia telangiectasia mutated homolog Antibody
Ataxia telangiectasia mutated homolog (human) Antibody
ATM (phospho S1981) Antibody
ATM (phospho S794) Antibody
ATM serine/threonine kinase Antibody
DNA PK Antibody
phospho S/T-Q Antibody
phospho SQ/TQ Antibody
Phospho SQ/TQ ATM/ATR Substrate Antibody
Serine protein kinase ATM Antibody
Serine-protein kinase ATM Antibody
Serine/threonine-protein kinase ATM Antibody
TEL1, telomere maintenance 1, homolog Antibody
Telomere fusion protein Antibody
Western blot analysis of extracts of CRC cells, using ATM antibody.
Immunohistochemistry of paraffin-embedded human pancreas using ATM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using ATM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ATM antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cell using ATM antibody.
Immunofluorescence analysis of CRC cell using ATM antibody.
Immunofluorescence analysis of Hela cell using ATM antibody.