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Anti-Apolipoprotein A1 Antibody

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ABclonal
A10739
Details
Description: Mouse monoclonal antibody to Apolipoprotein A1.
Category: Primary Antibodies
Applications: WB, IHC, IF
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P02647
Gene ID: 335
Immunogen: A synthetic peptide of human APOA1
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: apo(a)
Purification: Affinity purification
Background: This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:100

Target (Information from UniProt)
Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Tissue Specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Involvement in Disease: High density lipoprotein deficiency 2: Inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.

High density lipoprotein deficiency 1: Recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.

Amyloidosis 8: A form of hereditary generalized amyloidosis. Clinical features include extensive visceral amyloid deposits, renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. There is no involvement of the nervous system.
Sequence Similarities: Belongs to the apolipoprotein A1/A4/E family.
Post-Translational Modification: Glycosylated.
Cellular Location: Secreted.

Images
Western Blot - Anti-Apolipoprotein A1 Antibody from ABclonal (A10739) - Antibody Supply Service
Western blot analysis of extracts of HepG2 cells, using APOA1 antibody.

Product Code - A10739
Size Price Quantity
100µl £307
200µl £452

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