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Anti-HSP27 Antibody

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ABclonal
A10606
Overview
Name: Anti-HSP27 Antibody
Description: Mouse monoclonal antibody to HSP27.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200
Reactivity: Human
Immunogen: Recombinant protein of human HSPB1
Protein Length: 205
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Tissue Specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Involvement in Disease: Charcot-Marie-Tooth disease 2F: A dominant axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Onset of Charcot-Marie-Tooth disease type 2F is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later.

Neuronopathy, distal hereditary motor, 2B: A neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Sequence Similarities: Belongs to the small heat shock protein (HSP20) family.
Post-Translational Modification: Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).
Cellular Location: Cytoplasm. Nucleus. Cytoplasm > Cytoskeleton > Spindle.

Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
UniProt: P04792
Gene ID: 3315
Synonyms: Heat shock 27kDa protein Antibody
28 kDa heat shock protein Antibody
CMT2F Antibody
DKFZp586P1322 Antibody
DMPK-binding protein Antibody
epididymis secretory protein Li 102 Antibody
Estrogen regulated 24 kDa protein Antibody
Estrogen-regulated 24 kDa protein Antibody
Heat shock 25kDa protein 1 Antibody
Heat shock 27 kDa protein Antibody
Heat shock 27kD protein 1 Antibody
Heat shock 27kD protein 2 Antibody
Heat shock 27kDa protein 1 Antibody
Heat shock 27kDa protein 2 Antibody
Heat shock 28kDa protein 1 Antibody
Heat shock protein 1 Antibody
Heat shock protein 25 Antibody
Heat shock protein 25 kDa Antibody
Heat Shock Protein 27 Antibody
Heat shock protein beta 1 Antibody
Heat shock protein beta-1 Antibody
Heat shock protein beta-2 Antibody
heat shock protein family B (small) member 1 Antibody
Heat-shock protein beta-2 Antibody
HEL-S-102 Antibody
HMN2B Antibody
HS.76067 Antibody
Hs.78846 Antibody
Hsp 25 Antibody
HSP 27 Antibody
HSP 28 Antibody
Hsp B1 Antibody
Hsp25 Antibody
Hsp25 (phospho S78 + S82) Antibody
Hsp25 (phospho S78) Antibody
Hsp27 (phospho S15) Antibody
Hsp27 (phospho S78) Antibody
Hsp27 (phospho S82) Antibody
Hsp27 (phospho S85) Antibody
Hsp27 (phospho S86) Antibody
HSP28 Antibody
Hspb 1 Antibody
HspB1 Antibody
HSPB1_HUMAN Antibody
HSPB2 Antibody
HSPB2_HUMAN Antibody
LOH11CR1K Antibody
MGC133245 Antibody
MKBP Antibody
SRP 27 Antibody
SRP27 Antibody
Stress responsive protein 27 Antibody
Stress-responsive protein 27 Antibody

Images
Western Blot - Anti-HSP27 Antibody from ABclonal (A10606) - Antibody Supply Service
Western blot analysis of extracts of various cells, using HSPB1 antibody.

Product Code - A10606
Size Price Quantity
50µl £199
100µl £307
200µl £452

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