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Anti-Akt1 Antibody

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ABclonal
A10605
Overview
Name: Anti-Akt1 Antibody
Description: Mouse monoclonal antibody to Akt1.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:1000
Reactivity: Human
Immunogen: A synthetic peptide of human AKT1
Protein Length: 480
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.
Tissue Specificity: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Involvement in Disease: Breast cancer: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.

Colorectal cancer: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.

Proteus syndrome: A highly variable, severe disorder of asymmetric and disproportionate overgrowth of body parts, connective tissue nevi, epidermal nevi, dysregulated adipose tissue, and vascular malformations. Many features of Proteus syndrome overlap with other overgrowth syndromes.

Cowden syndrome 6: A form of Cowden syndrome, a hamartomatous polyposis syndrome with age-related penetrance. Cowden syndrome is characterized by hamartomatous lesions affecting derivatives of ectodermal, mesodermal and endodermal layers, macrocephaly, facial trichilemmomas (benign tumors of the hair follicle infundibulum), acral keratoses, papillomatous papules, and elevated risk for development of several types of malignancy, particularly breast carcinoma in women and thyroid carcinoma in both men and women. Colon cancer and renal cell carcinoma have also been reported. Hamartomas can be found in virtually every organ, but most commonly in the skin, gastrointestinal tract, breast and thyroid.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Post-Translational Modification: O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.
Cellular Location: Cytoplasm. Nucleus. Cell membrane.

Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529).
UniProt: P31749
Gene ID: 207
Synonyms: AKT Antibody
AKT 1 Antibody
AKT 2 Antibody
AKT1 (phospho S124) Antibody
AKT1 (phospho S129) Antibody
AKT1 (phospho S246) Antibody
AKT1 (phospho S473) Antibody
AKT1 (phospho T308) Antibody
AKT1 (phospho T34) Antibody
AKT1 (phospho T450) Antibody
AKT1 (phospho T72) Antibody
AKT1 (phospho Y326) Antibody
AKT1 kinase Antibody
AKT1/2 Antibody
AKT1/2/3 Antibody
AKT1/2/3 (phospho S472 + S473 + S474) Antibody
AKT1/2/3 (phospho Y315 + Y316 + Y312) Antibody
AKT1m Antibody
AKT1_HUMAN Antibody
AKT2 Antibody
AKT2 kinase Antibody
Akt3 Antibody
AKT3_HUMAN Antibody
C AKT Antibody
CAKT Antibody
CWS6 Antibody
DKFZp434N0250 Antibody
HIHGHH Antibody
kinase Akt1 Antibody
MGC99656 Antibody
MPPH Antibody
Murine thymoma viral (v akt) homolog 2 Antibody
Murine thymoma viral (v-akt) homolog 2 Antibody
Oncogene AKT1 Antibody
pan-AKT Antibody
pan-AKT (phospho T308) Antibody
PKB Antibody
PKB alpha Antibody
PKB beta Antibody
PKB gamma Antibody
PKB-ALPHA Antibody
PKB-GAMMA Antibody
PKB/Akt Antibody
PKBALPHA Antibody
PKBB Antibody
PKBBETA Antibody
PKBG Antibody
PKBGAMMA Antibody
PRKBA Antibody
PRKBB Antibody
PRKBG Antibody
Protein kinase Akt 2 Antibody
Protein kinase Akt-3 Antibody
Protein kinase B Antibody
Protein Kinase B Alpha Antibody
Protein kinase B beta Antibody
Protein kinase B gamma Antibody
Proto oncogene c Akt Antibody
Proto-oncogene c-Akt Antibody
RAC Antibody
RAC Alpha Antibody
RAC alpha serine/threonine protein kinase Antibody
RAC BETA Antibody
RAC beta serine threonine protein kinase Antibody
RAC beta serine/threonine protein kinase Antibody
RAC gamma Antibody
RAC PK alpha Antibody
RAC PK beta Antibody
RAC PK gamma Antibody
Rac protein kinase alpha Antibody
Rac protein kinase beta Antibody
RAC-alpha serine/threonine-protein kinase Antibody
RAC-gamma Antibody
RAC-gamma serine/threonine-protein kinase Antibody
RAC-PK-alpha Antibody
RAC-PK-gamma Antibody
RACALPHA Antibody
RACalpha serine/threonine kinase Antibody
RACbeta Antibody
RACgamma Antibody
RACgamma serine/threonine protein kinase Antibody
RACPKgamma Antibody
serine threonine protein kinase Antibody
STK-2 Antibody
STK2 Antibody
thymoma viral proto oncogene Antibody
thymoma viral proto oncogene 1 Antibody
v akt murine thymoma viral oncogene homolog 1 Antibody
v akt murine thymoma viral oncogene homolog 2 Antibody
V akt murine thymoma viral oncogene homolog 3 Antibody
V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) Antibody
vakt murine thymoma viral oncogene homolog 1 Antibody
vakt murine thymoma viral oncogene homolog 2 Antibody
vakt murine thymoma viral oncogene homolog 3 Antibody

Images
Western Blot - Anti-Akt1 Antibody from ABclonal (A10605) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using AKT1 antibody.

Product Code - A10605
Size Price Quantity
50µl £199
100µl £307
200µl £452

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