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Anti-SMURF2 Antibody

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ABclonal
A10592
Overview
Name: Anti-SMURF2 Antibody
Description: Rabbit polyclonal antibody to SMURF2.
Applications: WB
Dilutions: WB 1:1000 - 1:2000
Reactivity: Human
Immunogen: Recombinant protein of human SMURF2
Protein Length: 748
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Tissue Specificity: Widely expressed.
Post-Translational Modification: Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome.
Cellular Location: Nucleus. Cytoplasm. Cell membrane. Membrane raft.

Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
UniProt: Q9HAU4
Gene ID: 64750
Synonyms: E3 ubiquitin-protein ligase SMURF2 Antibody
EC 6.3.2. Antibody
hSMURF2 Antibody
MGC138150 Antibody
Smad specific E3 ubiquitin ligase 2 Antibody
SMAD specific E3 ubiquitin protein ligase 2 Antibody
SMAD ubiquitination regulatory factor 2 Antibody
SMAD-specific E3 ubiquitin-protein ligase 2 Antibody
SMUF2_HUMAN Antibody
SMURF 2 Antibody
Ubiquitin protein ligase SMURF2 Antibody

Images
Western Blot - Anti-SMURF2 Antibody from ABclonal (A10592) - Antibody Supply Service
Western blot analysis of extracts of SW480 cells, using SMURF2 antibody.

Product Code - A10592
Size Price Quantity
50µl £148
100µl £243
200µl £372

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