|Description:||Rabbit polyclonal antibody to SMURF2.|
|Dilutions:||WB 1:1000 - 1:2000|
|Immunogen:||Recombinant protein of human SMURF2|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.|
|Tissue Specificity:||Widely expressed.|
|Post-Translational Modification:||Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome.|
|Cellular Location:||Nucleus. Cytoplasm. Cell membrane. Membrane raft.
Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
|Synonyms:||E3 ubiquitin-protein ligase SMURF2 Antibody
EC 6.3.2. Antibody
Smad specific E3 ubiquitin ligase 2 Antibody
SMAD specific E3 ubiquitin protein ligase 2 Antibody
SMAD ubiquitination regulatory factor 2 Antibody
SMAD-specific E3 ubiquitin-protein ligase 2 Antibody
SMURF 2 Antibody
Ubiquitin protein ligase SMURF2 Antibody
Western blot analysis of extracts of SW480 cells, using SMURF2 antibody.