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Anti-KLF1 Antibody

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ABclonal
A10581
Details
Description: Rabbit polyclonal antibody to KLF1.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: Q13351
Gene ID: 10661
Immunogen: A synthetic peptide of human KLF1
Protein Length: 362
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: EKLF
Purification: Affinity purification
Background: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000

Target (Information from UniProt)
Function: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
Tissue Specificity: Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
Involvement in Disease: Anemia, congenital dyserythropoietic, 4: A blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.
Post-Translational Modification: Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity).
Cellular Location: Nucleus.

Colocalizes with SUMO1 in nuclear speckles.

Images
Western Blot - Anti-KLF1 Antibody from ABclonal (A10581) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using KLF1 antibody.

Product Code - A10581
Size Price Quantity
50µl £148
100µl £243
200µl £372

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