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Anti-USP28 Antibody

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ABclonal
A10400
Overview
Name: Anti-USP28 Antibody
Description: Rabbit polyclonal antibody to USP28.
Applications: WB
Dilutions: WB 1:500 - 1:2000
Reactivity: Human
Immunogen: Recombinant protein of human USP28
Protein Length: 1077
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
Sequence Similarities: Belongs to the peptidase C19 family. USP28 subfamily.
Post-Translational Modification: Degradaded upon nickel ion level or hypoxia exposure.
Cellular Location: Nucleus > Nucleoplasm.
UniProt: Q96RU2
Gene ID: 57646
Synonyms: Deubiquitinating enzyme 28 Antibody
KIAA1515 Antibody
Ubiquitin carboxyl terminal hydrolase 28 Antibody
Ubiquitin carboxyl-terminal hydrolase 28 Antibody
ubiquitin carboxyl-terminal hydrolase 28 variant 1 Antibody
Ubiquitin specific peptidase 28 Antibody
Ubiquitin specific processing protease 28 Antibody
Ubiquitin specific protease 28 Antibody
Ubiquitin thioesterase 28 Antibody
Ubiquitin thiolesterase 28 Antibody
Ubiquitin-specific-processing protease 28 Antibody
UBP28_HUMAN Antibody
USP 28 Antibody
USP28 protein Antibody

Images
Western Blot - Anti-USP28 Antibody from ABclonal (A10400) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using USP28 antibody.

Product Code - A10400
Size Price Quantity
50µl £148
100µl £243
200µl £372

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