phone icon
+44 (0)1223 298875

Anti-RBM4 Antibody

file icon Datasheet
ABclonal
A10362
Overview
Name: Anti-RBM4 Antibody
Description: Rabbit polyclonal antibody to RBM4.
Applications: WB
Dilutions: WB 1:500 - 1:2000
Reactivity: Human
Immunogen: Recombinant protein of human RBM4
Protein Length: 364
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
Tissue Specificity: Expressed in the cerebellum. Expressed in neurons and glial cells, including layers II neurons in the frontal cortex and CA1 pyramidal neurons in the hippocampus. Expressed in heart, liver, pancreas, skeletal muscle, placenta, primary fibroblasts and peripheral blood monocytes (at protein level). Ubiquitously expressed. Highly expressed in heart, placenta and skeletal muscle. Weakly expressed in pancreas, kidney, liver, lung and brain.
Post-Translational Modification: Phosphorylation on Ser-309 is induced upon cell muscle differentiation (By similarity). Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation.
Cellular Location: Nucleus. Nucleus > Nucleolus. Nucleus speckle. Cytoplasm. Cytoplasmic granule.

Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.
UniProt: Q9BWF3
Gene ID: 5936
Synonyms: dkfzp547k0918 Antibody
hLark Antibody
lark Antibody
Lark homolog Antibody
lark homologue Antibody
mgc75138 Antibody
rbm4 lark Antibody
RBM4_HUMAN Antibody
RNA binding motif 4 Antibody
RNA binding motif 4a Antibody
RNA-binding motif protein 4 Antibody
RNA-binding motif protein 4a Antibody
RNA-binding protein 4 Antibody

Images
Western Blot - Anti-RBM4 Antibody from ABclonal (A10362) - Antibody Supply Service
Western blot analysis of extracts of BT-474 cells, using RBM4 antibody.

Product Code - A10362
Size Price Quantity
50µl £148
100µl £243
200µl £372

or
Request a quotation
Contact us directly to order
  • call icon +44 (0)1223 298875
  • +44 (0)1223 281447
  • email icon orders@ab-y-ss.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe