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Anti-EGLN1/EGLN2 Antibody

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Name: Anti-EGLN1/EGLN2 Antibody
Description: Rabbit polyclonal antibody to EGLN1/EGLN2.
Applications: WB
Dilutions: WB 1:500 - 1:2000
Reactivity: Human
Immunogen: A synthetic peptide of human EGLN1/EGLN2
Protein Length: 426aa/407aa
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
Tissue Specificity: According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle.
Involvement in Disease: Erythrocytosis, familial, 3: An autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
Post-Translational Modification: S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326.
Cellular Location: Cytoplasm. Nucleus.

Mainly cytoplasmic. Shuttles between the nucleus and cytoplasm (PubMed:19631610). Nuclear export requires functional XPO1.
UniProt: Q9GZT9 / Q96KS0
Gene ID: 54583 / 112398
Synonyms: C1ORF12 Antibody
Chromosome 1 Open Reading Frame 12 Antibody
DKFZp761F179 Antibody
ECYT 3 Antibody
ECYT3 Antibody
EGL 9 homolog of C. elegans 1 Antibody
EGL nine (C.elegans) homolog 1 Antibody
Egl nine homolog 1 Antibody
Egl nine homolog 1 (C. elegans) Antibody
Egl nine like protein 1 Antibody
EGLN 1 Antibody
EGLN1_HUMAN Antibody
HIF PH2 Antibody
HIF Prolyl Hydroxylase 2 Antibody
HIF-PH2 Antibody
HIF-prolyl hydroxylase 2 Antibody
HIFP4H 2 Antibody
HIFPH2 Antibody
HPH 2 Antibody
HPH-2 Antibody
HPH2 Antibody
Hypoxia inducible factor prolyl hydroxylase 2 Antibody
Hypoxia-inducible factor prolyl hydroxylase 2 Antibody
ORF13 Antibody
P4H2 Antibody
PHD 2 Antibody
PhD2 Antibody
PHD2 / prolyl hydroxylase Antibody
PNAS 118 Antibody
PNAS 137 Antibody
Prolyl Hydroxylase Domain Containing Protein 2 Antibody
Prolyl hydroxylase domain-containing protein 2 Antibody
Rat Homolog of SM20 Antibody
SM 20 Antibody
SM-20 Antibody
SM20 Antibody
Zinc finger MYND domain containing protein 6 Antibody
ZMYND6 Antibody

Product Code - A10342
Size Price Quantity
50µl £148
100µl £243
200µl £372

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