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Anti-CPSF4 Antibody

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ABclonal
A10284
Details
Description: Rabbit polyclonal antibody to CPSF4.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: O95639
Gene ID: 10898
Immunogen: Recombinant protein of human CPSF4
Protein Length: 269 (244)
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: NAR; NEB1; NEB-1; CPSF30
Purification: Affinity purification
Background: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:1000 - 1:2000

Target (Information from UniProt)
Function: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Sequence Similarities: Belongs to the CPSF4/YTH1 family.
Cellular Location: Nucleus.

Images
Western Blot - Anti-CPSF4 Antibody from ABclonal (A10284) - Antibody Supply Service
Western blot analysis of extract of various cells, using CPSF4 antibody.

Product Code - A10284
Size Price Quantity
50µl £148
100µl £243
200µl £372

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