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Anti-RBBP8 Antibody

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Name: Anti-RBBP8 Antibody
Description: Rabbit polyclonal antibody to RBBP8.
Applications: WB
Dilutions: WB 1:500 - 1:2000
Reactivity: Human
Immunogen: Recombinant protein of human RBBP8
Protein Length: 897
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).
Tissue Specificity: Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).
Involvement in Disease: Seckel syndrome 2: A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.

Jawad syndrome: A syndrome characterized by congenital microcephaly, moderately severe mental retardation, and symmetrical digital anomalies. Digital malformations of variable degree include hallux valgus, syndactyly of toes 4 and 5, short fifth fingers, single flexion crease of fifth fingers, polydactyly and synpolydactyly.
Sequence Similarities: Belongs to the COM1/SAE2/CtIP family.
Post-Translational Modification: Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
Cellular Location: Nucleus. Chromosome.

Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, Ref.29). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
UniProt: Q99708
Gene ID: 5932
Synonyms: COM1 Antibody
COM1_HUMAN Antibody
CtBP interacting protein Antibody
CtBP-interacting protein Antibody
CtIP Antibody
DNA endonuclease RBBP8 Antibody
JWDS Antibody
RBBP-8 Antibody
Retinoblastoma-binding protein 8 Antibody
Retinoblastoma-interacting protein and myosin-like Antibody
Rim Antibody
SAE2 Antibody
SCKL2 Antibody
Sporulation in the absence of SPO11 protein 2 homolog Antibody

Western Blot - Anti-RBBP8 Antibody from ABclonal (A10201) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using RBBP8 antibody.

Product Code - A10201
Size Price Quantity
50µl £148
100µl £243
200µl £372

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