|Description:||Rabbit polyclonal antibody to NPC1L1.|
|Immunogen:||Recombinant protein of human NPC1L1|
|Protein Length:||1359 (1332)|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:1000 - 1:4000|
Target (Information from UniProt)
|Function:||Plays a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation.|
|Tissue Specificity:||Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle.|
|Sequence Similarities:||Belongs to the patched family.|
|Post-Translational Modification:||Highly glycosylated.|
|Cellular Location:||Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane.
Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.
Western blot analysis of extracts of various cells, using NPC1L1 antibody.