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Anti-CDC25A Antibody

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ABclonal
A0896
Overview
Name: Anti-CDC25A Antibody
Description: Rabbit polyclonal antibody to CDC25A.
Applications: WB
Dilutions: WB 1:500 - 1:2000
Reactivity: Human
Immunogen: A synthetic peptide of human CDC25A
Protein Length: 524
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence Similarities: Belongs to the MPI phosphatase family.
Post-Translational Modification: Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
UniProt: P30304
Gene ID: 993
Synonyms: Cdc 25a Antibody
Cdc25A (phospho S124) Antibody
Cdc25A (phospho S178) Antibody
Cdc25A (phospho S278) Antibody
Cdc25A (phospho S75) Antibody
Cdc25A (phospho S76) Antibody
Cdc25A (phospho T507) Antibody
CDC25A2 Antibody
CDC25A2 CAG isoform Antibody
Cell division cycle 25 homolog A (S. pombe) Antibody
Cell division cycle 25A Antibody
Cell division cycle 25A isoform a Antibody
Cell division cycle 25A isoform b Antibody
D9Ertd393e Antibody
Dual specificity phosphatase Cdc25A Antibody
M phase inducer phosphatase 1 Antibody
M-phase inducer phosphatase 1 Antibody
MGC115549 Antibody
MPIP1_HUMAN Antibody

Images
Western Blot - Anti-CDC25A Antibody from ABclonal (A0896) - Antibody Supply Service
Western blot analysis of extracts of NIH-3T3 cell line, using CDC25A antibody.

Product Code - A0896
Size Price Quantity
100µl £243
200µl £372

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