|Description:||Rabbit polyclonal antibody to IRAK1.|
|Dilutions:||WB 1:500 - 1:1000, IHC 1:50 - 1:100|
|Immunogen:||A synthetic peptide of human IRAK1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.|
|Tissue Specificity:||Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.|
|Sequence Similarities:||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.|
|Post-Translational Modification:||Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.|
|Cellular Location:||Cytoplasm. Nucleus. Lipid droplet.
Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity).
Interleukin 1 receptor associated kinase 1 Antibody
Interleukin-1 receptor-associated kinase 1 Antibody
IRAK (phospho T100) Antibody
IRAK (phospho T387) Antibody
Pelle homolog Antibody
Pelle-like protein kinase Antibody
Western blot analysis of extracts of various cell lines, using IRAK1 antibody.