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Anti-YY1 Antibody

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ABclonal
A0856
Details
Description: Rabbit polyclonal antibody to YY1.
Category: Primary Antibodies
Applications: WB, IHC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P25490
Gene ID: 7528
Immunogen: A synthetic peptide of human YY1
Protein Length: 414
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
Purification: Affinity purification
Background: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, IHC 1:20 - 1:50

Target (Information from UniProt)
Function: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).
Sequence Similarities: Belongs to the YY transcription factor family.
Post-Translational Modification: Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.
Cellular Location: Nucleus matrix.

Associated with the nuclear matrix.

Images
Western Blot - Anti-YY1 Antibody from ABclonal (A0856) - Antibody Supply Service
Western blot analysis of extracts of Y79 cell line, using YY1 antibody.

Product Code - A0856
Size Price Quantity
100µl £243
200µl £372

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