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Anti-AIF Antibody

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ABclonal
A0811
Overview
Name: Anti-AIF Antibody
Description: Rabbit polyclonal antibody to AIF.
Applications: WB, IHC, IF, FC
Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:200, IF 1:20 - 1:50, FC 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human AIFM1
Protein Length: 613
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Tissue Specificity: Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Involvement in Disease: Combined oxidative phosphorylation deficiency 6: A mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting. Some patients manifest prenatal ventriculomegaly and severe postnatal encephalomyopathy.

Cowchock syndrome: An X-linked recessive neuromuscular disorder characterized by early childhood onset of a slowly progressive axonal sensorimotor neuropathy associated in some patients with sensorineural deafness and cognitive impairment.

Deafness, X-linked, 5: A form of hearing loss characterized by absent or severely abnormal auditory brainstem response, abnormal middle ear reflexes, abnormal speech discrimination, loss of outer hair cell function, and cochlear nerve hypoplasia. DFNX5 patients manifest auditory neuropathy with childhood onset, associated with distal sensory impairment affecting the peripheral nervous system.
Sequence Similarities: Belongs to the FAD-dependent oxidoreductase family.
Post-Translational Modification: Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Cellular Location: Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > Perinuclear region.

Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
UniProt: O95831
Gene ID: 9131
Synonyms: AIFM1 Antibody
AIFM1_HUMAN Antibody
Apoptosis inducing factor Antibody
Apoptosis inducing factor 1, mitochondrial Antibody
Apoptosis inducing factor, mitochondrion associated, 1 Antibody
Apoptosis-inducing factor 1 Antibody
CMTX4 Antibody
COWCK Antibody
COXPD6 Antibody
Harlequin Antibody
Hq Antibody
mAIF Antibody
MGC111425 Antibody
MGC5706 Antibody
mitochondrial Antibody
Neuropathy, axonal motor-sensory, with deafness and mental retardation Antibody
neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) Antibody
PDCD 8 Antibody
PDCD8 Antibody
Programmed cell death 8 Antibody
Programmed cell death 8 (apoptosis inducing factor) Antibody
Programmed cell death 8 isoform 1 Antibody
Programmed cell death 8 isoform 2 Antibody
Programmed cell death 8 isoform 3 Antibody
Programmed cell death protein 8 Antibody
Programmed cell death protein 8 mitochondrial Antibody
Programmed cell death protein 8 mitochondrial precursor Antibody
Striatal apoptosis inducing factor Antibody

Images
Western Blot - Anti-AIF Antibody from ABclonal (A0811) - Antibody Supply Service
Western blot analysis of extracts of HepG2 cells tissue, using AIFM1 antibody.

Product Code - A0811
Size Price Quantity
100µl £243
200µl £372

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