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Anti-ATF-2 Antibody

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ABclonal
A0757
Overview
Name: Anti-ATF-2 Antibody
Description: Rabbit polyclonal antibody to ATF-2.
Applications: WB, IHC, ICC, IP
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100, ICC 1:50 - 1:200, IP 1:20 - 1:50
Reactivity: Human
Immunogen: Recombinant protein of human ATF2
Protein Length: 505
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
Tissue Specificity: Ubiquitously expressed, with more abundant expression in the brain.
Sequence Similarities: Belongs to the bZIP family. ATF subfamily.
Post-Translational Modification: Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity.
Cellular Location: Nucleus. Cytoplasm. Mitochondrion outer membrane.

Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
UniProt: P15336
Gene ID: 1386
Synonyms: Activating transcription factor 2 Antibody
Activating transcription factor 2 splice variant ATF2 var2 Antibody
ATF 2 Antibody
ATF2 Antibody
ATF2 (phospho S472) Antibody
ATF2 (phospho S480) Antibody
ATF2 (phospho S490 + S498) Antibody
ATF2 (phospho S490) Antibody
ATF2 (phospho S62 + S44) Antibody
ATF2 (phospho S94) Antibody
ATF2 (phospho T51 + T53) Antibody
ATF2 (phospho T52) Antibody
ATF2 (phospho T69 + T51) Antibody
ATF2 (phospho T69 + T71) Antibody
ATF2 (phospho T71 + T53) Antibody
ATF2 (phospho T71) Antibody
ATF2 (phospho T73 + T55) Antibody
ATF2 protein Antibody
ATF2_HUMAN Antibody
cAMP Response Element Binding Protein 2 Antibody
cAMP response element binding protein CRE BP1 Antibody
cAMP response element-binding protein CRE-BP1 Antibody
cAMP responsive element binding protein 2, formerly Antibody
cAMP-dependent transcription factor ATF-2 Antibody
cAMP-responsive element-binding protein 2 Antibody
CRE BP1 Antibody
CRE-BP Antibody
CREB 2 Antibody
CREB-2 Antibody
CREB2 Antibody
CREBP1 Antibody
Cyclic AMP dependent transcription factor ATF 2 Antibody
Cyclic AMP-dependent transcription factor ATF-2 Antibody
Cyclic AMP-responsive element-binding protein 2 Antibody
D130078H02Rik Antibody
D18875 Antibody
HB 16 Antibody
HB16 Antibody
Histone acetyltransferase ATF2 Antibody
MGC105211 Antibody
MGC105222 Antibody
MGC111558 Antibody
MGC142504 Antibody
mXBP Antibody
MXBP protein Antibody
Tg(Gzma-Klra1)7Wum Antibody
TREB 7 Antibody
TREB7 Antibody

Images
Western Blot - Anti-ATF-2 Antibody from ABclonal (A0757) - Antibody Supply Service
Western blot analysis of extracts of HeLa cell line, using ATF2 antibody.

Product Code - A0757
Size Price Quantity
50µl £243
100µl £372
200µl £565

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