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Anti-BAD Antibody

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ABclonal
A0741
Overview
Name: Anti-BAD Antibody
Description: Rabbit polyclonal antibody to BAD.
Applications: WB, IHC
Dilutions: WB 1:100 - 1:200, IHC 1:50 - 1:100
Reactivity: Human
Immunogen: A synthetic peptide of human Bad
Protein Length: 168
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Tissue Specificity: Expressed in a wide variety of tissues.
Sequence Similarities: Belongs to the Bcl-2 family.
Post-Translational Modification: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.
Cellular Location: Mitochondrion outer membrane. Cytoplasm.

Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm.
UniProt: Q92934
Gene ID: 572
Synonyms: AI325008 Antibody
Bad (phospho S112) Antibody
Bad (phospho S118) Antibody
Bad (phospho S134) Antibody
Bad (phospho S136) Antibody
Bad (phospho S155) Antibody
Bad (phospho S75) Antibody
Bad (phospho S91) Antibody
Bad (phospho S99) Antibody
BAD_HUMAN Antibody
BBC 2 Antibody
BBC2 Antibody
BBC6 Antibody
Bcl 2 Antagonist of Cell Death Antibody
Bcl 2 Binding Component 6 Antibody
BCL X / BCL 2 Binding Protein Antibody
BCL X Binding Protein Antibody
Bcl XL/Bcl 2 Associated Death Promoter Antibody
Bcl-2-binding component 6 Antibody
Bcl-2-like protein 8 Antibody
Bcl-XL/Bcl-2-associated death promoter Antibody
Bcl2 antagonist of cell death Antibody
BCL2 antagonist of cell death protein Antibody
BCL2 associated agonist of cell death Antibody
Bcl2 Associated Death Promoter Antibody
BCL2 binding component 6 Antibody
BCL2 binding protein Antibody
Bcl2 Like 8 Protein Antibody
Bcl2-L-8 Antibody
BCL2L8 Antibody
Proapoptotic BH3 Only Protein Antibody

Images
Western Blot - Anti-BAD Antibody from ABclonal (A0741) - Antibody Supply Service
Western blot analysis of extracts of various cells, using BAD antibody.

Product Code - A0741
Size Price Quantity
50µl £148
100µl £243
200µl £372

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