Anti-Topoisomerase IIα AntibodyDatasheet
|Name:||Anti-Topoisomerase IIα Antibody|
|Description:||Rabbit polyclonal antibody to Topoisomerase IIα.|
|Applications:||WB, IHC, IF|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:50|
|Immunogen:||A synthetic peptide of human TOP2A|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).|
|Sequence Similarities:||Belongs to the type II topoisomerase family.|
|Post-Translational Modification:||Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.|
|Cellular Location:||Cytoplasm. Nucleus > Nucleoplasm.
Generally located in the nucleoplasm.
Western blot analysis of extracts of various cell lines, using TOP2A antibody.