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Anti-HMGB1 Antibody

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ABclonal
A0718
Overview
Name: Anti-HMGB1 Antibody
Description: Rabbit polyclonal antibody to HMGB1.
Applications: WB, FC
Dilutions: WB 1:500 - 1:2000, FC 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human HMGB1
Protein Length: 215
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).
Tissue Specificity: Ubiquituous. Expressed in platelets (PubMed:11154118).
Sequence Similarities: Belongs to the HMGB family.
Post-Translational Modification: Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Cellular Location: Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane. Endosome. Endoplasmic reticulum-Golgi intermediate compartment.

In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118).
UniProt: P09429
Gene ID: 3146
Synonyms: Amphoterin Antibody
Chromosomal protein, nonhistone, HMG1 Antibody
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Images
Western Blot - Anti-HMGB1 Antibody from ABclonal (A0718) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using HMGB1 antibody.

Product Code - A0718
Size Price Quantity
100µl £243
200µl £372

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