|Description:||Rabbit polyclonal antibody to TJP1.|
|Applications:||WB, IHC, IF, IP, FC|
|Immunogen:||Recombinant Protein of human TJP1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||TJP1; DKFZp686M05161; MGC133289; ZO-1|
|Background:||Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:200 - 1:500, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50, FC 1:20 - 1:50|
Target (Information from UniProt)
|Function:||The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.|
|Tissue Specificity:||The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.|
|Sequence Similarities:||Belongs to the MAGUK family.|
|Post-Translational Modification:||Phosphorylated. Dephosphorylated by PTPRJ.|
|Cellular Location:||Cell membrane. Cell junction > Tight junction. Cell junction. Cell junction > Gap junction.
Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells.
Western blot analysis of extracts of various cell lines, using TJP1 antibody.