|Description:||Rabbit polyclonal antibody to Mad-1.|
|Dilutions:||WB 1:500 - 1:1000|
|Immunogen:||A synthetic peptide of human MAD1L1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.|
|Tissue Specificity:||Expressed weakly at G0/G1 and highly at late S and G2/M phase.|
|Sequence Similarities:||Belongs to the MAD1 family.|
|Post-Translational Modification:||Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.|
|Cellular Location:||Nucleus. Chromosome > Centromere > Kinetochore. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Cytoplasm > Cytoskeleton > Spindle.
From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
Western blot analysis of extracts of A431 cells tissue, using MAD1L1 antibody.