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Anti-Profilin-1 Antibody

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ABclonal
A0639
Details
Description: Rabbit polyclonal antibody to Profilin-1.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P07737
Gene ID: 5216
Immunogen: A synthetic peptide of human PFN1
Protein Length: 140
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: ALS18
Purification: Affinity purification
Background: This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000

Target (Information from UniProt)
Function: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Tissue Specificity: Expressed in epididymis (at protein level).
Involvement in Disease: Amyotrophic lateral sclerosis 18: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
Sequence Similarities: Belongs to the profilin family.
Post-Translational Modification: Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.
Cellular Location: Cytoplasm > Cytoskeleton.

Images
Western Blot - Anti-Profilin-1 Antibody from ABclonal (A0639) - Antibody Supply Service
Western blot analysis of extracts of HeLa cells, using PFN1 antibody.

Product Code - A0639
Size Price Quantity
100µl £243
200µl £372

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