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Anti-BLNK Antibody

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ABclonal
A0638
Overview
Name: Anti-BLNK Antibody
Description: Mouse monoclonal antibody to BLNK.
Applications: WB
Dilutions: WB 1:500 - 1:1000
Reactivity: Human
Immunogen: A synthetic peptide of human BLNK
Protein Length: 456
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Tissue Specificity: Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
Involvement in Disease: Agammaglobulinemia 4, autosomal recessive: A primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
Post-Translational Modification: Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
Cellular Location: Cytoplasm. Cell membrane.

BCR activation results in the translocation to membrane fraction.
UniProt: Q8WV28
Gene ID: 29760
Synonyms: AGM4 Antibody
B cell adapter containing SH2 domain protein Antibody
B cell adapter containing Src homology 2 domain protein Antibody
B cell adaptor containing SH2 domain Antibody
B cell linker Antibody
B cell linker protein Antibody
B cell-specific adaptor protein Antibody
B-cell activation Antibody
B-cell adapter containing a SH2 domain protein Antibody
B-cell adapter containing a Src homology 2 domain protein Antibody
B-cell linker protein Antibody
BASH Antibody
Bca Antibody
BLNK (phospho Y84) Antibody
BLNK (phospho Y96) Antibody
BLNK s Antibody
BLNK_HUMAN Antibody
Cytoplasmic adapter protein Antibody
Ly 57 Antibody
Ly57 Antibody
Lymphocyte antigen 57 Antibody
Lyw 57 Antibody
Lyw57 Antibody
MGC111051 Antibody
SH2 domain-containing leukocyte protein, 65-KD Antibody
SLP 65 Antibody
SLP-65 Antibody
SLP65 Antibody
Src homology 2 domain containing leukocyte protein of 65 kDa Antibody
Src homology 2 domain-containing leukocyte protein of 65 kDa Antibody
Src homology [SH2] domain-containing leukocyte protein of 65 kD Antibody

Images
Western Blot - Anti-BLNK Antibody from ABclonal (A0638) - Antibody Supply Service
Western blot analysis of extracts of NCI-H460 cell line, using BLNK antibody.

Product Code - A0638
Size Price Quantity
100µl £243
200µl £372

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